本實(shí)驗(yàn)的目的是評價(jià)不同過氧化水平的大豆油（SO）對保育豬脂質(zhì)、N、GE消化率、腸道完整性、氧化應(yīng)激及生長性能的影響。日糧處理組包括10%的新鮮SO（22.5℃處理），或者10%熱處理SO（45℃加熱288 h，90℃加熱72 h，或者180℃加熱6 h），每一處理都進(jìn)行15 L / min的空氣注入；處理后過氧化值分別為7.6、11.5、19.1和13.4 mEq/kg，混合型值分別為1.92、6.29、149和159。
在試驗(yàn)1中，64頭仔豬（初始體重（BW）7.1±0.9 kg）被隨機(jī)分配到2個(gè)飼養(yǎng)舍32欄中，分別飼喂為期21 d的試驗(yàn)飼糧，第20天采集新鮮糞便樣品，測定GE和脂質(zhì)消化率。在試驗(yàn)2中，將56頭仔豬 （BW 9.16±1.56 kg）放入單獨(dú)的代謝籠中，以評估GE、脂質(zhì)、N的消化率和N的保留率；檢測尿液中乳果糖與甘露醇的比例，以評估體內(nèi)小腸的完整性；收集尿液和血漿進(jìn)行氧化應(yīng)激標(biāo)記物的分析；隨后對豬實(shí)施安樂死以獲得肝臟重量，并分析肝臟中氧化應(yīng)激標(biāo)記物。
試驗(yàn)數(shù)據(jù)顯示，試驗(yàn)1中，與其他SO處理組相比 90℃熱處理的SO組豬只的ADG（P = 0.01）和ADFI（P = 0.04）顯著降低，而22.5℃、45℃和180℃熱處理的各SO組豬只無差異；各組間日糧GE和脂質(zhì)消化率沒有差異（P > 0.10）。在試驗(yàn)2中，各過氧化SO組間，DE：ME比值、N消化率、N的保留或者尿液中乳果糖與甘露醇比值，血清、尿液和肝臟中硫代巴比妥酸反應(yīng)性物質(zhì)，以及血漿蛋白質(zhì)羰基、尿液和肝臟8-OH-2dG等指標(biāo)均沒有顯著差異（P > 0.10）；與新鮮SO組比較，90℃熱處理SO組豬只血清（P ≤ 0.01）和尿液（P ≤ 0.05）中異前列腺素濃度最大。
這些結(jié)果表明，過氧化SO中脂肪酸組成的變化和/或脂質(zhì)過氧化產(chǎn)物的存在可能會(huì)降低保育豬的ADG和ADFI；但好像對GE、脂質(zhì)或N消化率，以及腸道通透性沒有影響。這些數(shù)據(jù)表明，脂質(zhì)過氧化產(chǎn)物的存在可能影響氧化應(yīng)激的某些標(biāo)志物。

Influence of feeding thermally peroxidized soybean oil on growth performance, digestibility, gut integrity, and oxidative stress in nursery pigs
The objectives of the current experiments were to evaluate the effect of feeding soybean oil (SO) with different levels of peroxidation on lipid, N, and GE digestibility, gut integrity, oxidative stress, and growth performance in nursery pigs. Treatments consisted diets containing 10% fresh SO (22.5 °C) or thermally processed SO (45 °C for 288 h, 90 °C for 72 h, or 180 °C for 6 h), each with an air infusion of 15 L/min, with postprocessing peroxide values of 7.6, 11.5, 19.1, and 13.4 mEq/kg and p-anisidine values of 1.92, 6.29, 149, and 159, for the 22.5 °C, 45 °C, 90 °C and 180 °C processed SO, respectively. In experiment 1, 64 barrows (7.1 ± 0.9 kg initial BW) were randomly allotted into 2 rooms of 32 pens and individually fed their experimental diets for 21 d, with a fresh fecal sample collected on day 20 for determination of GE and lipid digestibility. In experiment 2, 56 barrows (BW 9.16 ± 1.56 kg) were placed into individual metabolism crates for assessment of GE, lipid, andN digestibility and N retention. Urinary lactulose to mannitol ratio was assessed to evaluate in vivo small intestinal integrity, and urine and plasma were collected to analyze for markers of oxidative stress. Pigs were subsequently euthanized to obtain liver weights and analyze the liver for markers of oxidative stress. In experiment 1, pigs fed the SO thermally processed at 90 °C had reduced ADG (P = 0.01) and ADFI (P = 0.04) compared to pigs fed the other SO treatment groups, with no differences noted among pigs fed the 22.5 °C, 45 °C, and 180 °C SO treatments. No effects of feeding thermally processing SO on dietary GE or lipid digestibility (P > 0.10) were noted in either experiment. In experiment 2, there was no dietary effect of feeding peroxidized SO on the DE:ME ratio, N digestibility, or N retained as a percent of N digested, on the urinary ratio of lactulose to mannitol, on serum, urinary, or liver thiobarbituric acid reactive substances, on plasma protein carbonyls, or on urinary or liver 8-OH-2dG (P > 0.10). In experiment 2, pigs fed the SO thermally processed at 90 °C had the greatest isoprostane concentrations in the serum (P ≤ 0.01) and urine (P ≤ 0.05) compared to pigs fed the unprocessed SO. These results indicate that the change in fatty acid composition and/or the presence of lipid peroxidation products in peroxidized SO may reduce ADG and ADFI in nursery pigs, but appears to have no impact on GE, lipid, or N digestibility, or gut permeability. These data suggest that the presence of lipid peroxidation products may affect certain markers of oxidative stress.

文章來源：豬營養(yǎng)國際論壇
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